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Monday, January 21, 2013
Note for C-P ELISA Kit
C-P ELISA Kit apprehension kit for accurate research. Human C polypeptide ( CP ) aqueous samples ELISA Kit: including serum, plasma, urine, pleural effusion, cerebrospinal fluid, corpuscle ability supernatant. Serum: agglomeration at allowance temperature for 10-20 minutes, and centrifuged for 20 account or so ( 2000-3000 R / min ). Afloat carefully. If precipitation in the canning process, centrifugal again. Plasma: should be based on the requirements of the case EDTA, sodium citrate or heparin as an anticoagulant, 10-20 account afterwards mixing, centrifuged for 20 account or so ( 2000-3000 R / min ). Afloat carefully. If precipitation in the canning process, centrifugal again. Urine: a antiseptic collection. Centrifuge for 20 account ( 2000-3000 rpm ). Afloat carefully. If precipitation in the canning process, centrifugal again. Pleural effusion, cerebrospinal aqueous with advertence to this practice. The afloat of corpuscle culture: the apprehension of secretory component, calm with antiseptic tube. Centrifuge for 20 account ( 2000-3000 rpm ). Afloat carefully.
C-P ELISA Kit is removed from the air-conditioned ambiance should be in calm at allowance temperature for 15-30 account before, ELISA plates coated in Kaifeng such as unused, bowl should be stored in closed bag. Concentrated abrasion aqueous may be crystallized, concoction can be acrimonious the baptize solubilization, abrasion does not affect the results. Each footfall of sample should be application a pipette, and generally affidavit of its accuracy, to abstain analysis error. A sample time best ascendancy in 5 minutes, such as case number, acclaim to use advance sample. Each altitude at the aforementioned time as the accepted curve, the best do circuitous hole. If the specimens testing actual agreeable is too top ( the sample od is bigger than the aboriginal accepted aperture aperture OD), amuse use the sample concoction assorted of concoction ( n times) and again were calculated, if you assuredly assorted by the absolute concoction ( * n * 5 ). Closure bowl film alone a ancient use, to abstain cantankerous contamination. The substrate stored. The substrate stored.
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